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  • Nanopore bioinformatics & EPI2ME

    Hello! I've just started a PhD in DNA sequencing for the identification of cancer biomarkers and I was hoping to get some advice on bioinformatic methods. Part of my experimental design is to carry out Nanopore sequencing and I'm quite new to long-read sequencing and bioinformatics. I'd originally planned to use the 'easy' bioinformatic pipeline as advertised by Nanopore so I could practice/learn command line but not miss out on sequencing samples, however I've also noticed its not common to see it in journals or discussions.

    Why is this? Would I be unable to publish data analysed with epi2me? Is it better if I sequence the samples THEN carry out bioinformatic analysis when I'm more confident in it?

    Thanks!!

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  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    Yesterday, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR
    by seqadmin



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM

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