Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • veena
    Junior Member
    • Feb 2009
    • 9

    #1

    chord->getLength Newbler error

    Hi,

    I'm trying to run a Newbler assembly on a sample that is predicted to be ~8-9Mb but get the following error message:

    Error: An internal error has occurred in the computation.
    Assertion: chord->getLength() > 10
    Location: 'bool ChordMatrix::checkAddChordColumn(Chord*, int)'
    [ChordMatrix.cpp:182]
    Please report this error to your customer support representative.

    I was wondering if this rings a bell to anybody and if you knew how to fix it.

    Thanks!
  • Torst
    Senior Member
    • Apr 2008
    • 275

    #2
    I experienced this error a lot when using FASTA file input rather than SFF files. This was with Version 1 of Newbler. If you can get Version 2 of Newbler, try that.

    Comment

    • plattsa
      Member
      • Mar 2009
      • 17

      #3
      I still get this error with FASTA files in 2.3, no real help from Roche when I contacted them.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      43 views
      0 reactions
      Last Post SEQadmin2  
      Working...