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  • allele-specific expression by direct genome and transcritpome sequencing

    We sequenced the diploid genome of a single individual and their transcritome.
    (Poplar)

    Fisrt, we identiy all the heterozygosity SNPs (genome).

    If i know it's a heterozygosity SNPs, Should I do a chi square test using the number (65,89,38,28) or do a bionomal test (50,50,38,28) ?

    an example of heterozygosity SNP (The numbers are counts of the two alleles observed in gDNA or cDNA)

    gDNA cDNA
    A 65 38
    G 89 28

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