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  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #166
    Post a few lines from clc_trim_pair4.fq. Are you getting that error right away?

    Code:
    $ head -6 clc_trim_pair4.fq

    Comment

    • kaps
      Member
      • Jan 2015
      • 71

      #167
      Originally posted by GenoMax View Post
      Post a few lines from clc_trim_pair4.fq. Are you getting that error right away?

      Code:
      $ head -6 clc_trim_pair4.fq
      This is what I get:
      @No_name
      CAACATTACCTAGTGCAAGACAGTGTTAATTTGAAGGCAGTTCATA
      +
      IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
      @No_name
      TTTTTTTTTTTTTTTTCTTTTCTCAACTTCCTTCACCTTCACACTCTCTTTCCCTTCTTC

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #168
        Is that all the sequence in the file? Just one record?

        If not, are all sequences called "NO_name"? That is not going to work since the names are not unique.

        Post output of.

        Code:
        $ ls -lh clc_trim_pair4.fq

        Comment

        • sandia
          Junior Member
          • Jun 2015
          • 3

          #169
          Hi all,
          I've been trying to download Bowtie2.2.5 for Mac and followed instructions on this forum. The consistent error message I've been getting is: (ERR): Expected bowtie2 to be in same directory with bowtie2-align: /Applications/bowtie2-2.2.5/
          I've followed suggestions from others with this problem to address this and I've realized that bowtie2-align is not in the folder I am downloading. Is this normal? Do I need to retrieve it elsewhere? I assume I am missing something basic since I am new to all this. Thanks in advance for any help.

          Comment

          • GenoMax
            Senior Member
            • Feb 2008
            • 7142

            #170
            bowtie2 web site appears to be experiencing issues at the moment (can't get to the 2.2.6 code). Are you downloading the pre-compiled binary zip file for OS X from this link: http://sourceforge.net/projects/bowt...owtie2/2.2.5/? After uncompressing the archive all files you need should be in the uncompressed folder.

            Comment

            • sandia
              Junior Member
              • Jun 2015
              • 3

              #171
              Thanks for the reply. Yes I've downloaded that file but it does not contain bowtie2-align.

              Comment

              • GenoMax
                Senior Member
                • Feb 2008
                • 7142

                #172
                I just checked the zip archive and the executables are all there (there are multiple align versions, bowtie2-align-l,bowtie2-align-s etc). You should run bowtie2 like so

                Code:
                $ bowtie2 -help

                Comment

                • sandia
                  Junior Member
                  • Jun 2015
                  • 3

                  #173
                  Got it, thank you!

                  Comment

                  • delphin
                    Junior Member
                    • Jul 2021
                    • 1

                    #174
                    error message: bowtie2 installation on mac

                    Hello, I'm not familiar to informatics and I try to install Bowtie2 on mac. I download bowtie2 source file.zip
                    I unzip it then when I did 'make' I have this error message:

                    1 error generated.
                    In file included from aligner_swsse_ee_u8.cpp:56:
                    In file included from ./aligner_sw.h:73:
                    ./sse_wrap.h:31:10: fatal error: 'simde/x86/sse2.h' file not found
                    #include "simde/x86/sse2.h"

                    Could you help me.

                    Thank you

                    Comment

                    • GenoMax
                      Senior Member
                      • Feb 2008
                      • 7142

                      #175
                      Download pre-compiled binary for macOS from here.

                      Consider using "conda" to install these programs that will save you a lot of time/headache.

                      Comment

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