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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

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Started by laura, 05-09-2011, 04:42 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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Started by sc_123, 04-09-2018, 02:25 AM
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Started by bong28, 04-08-2018, 10:27 AM
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Started by ETHANol, 03-23-2012, 10:30 PM
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Started by dkumar, 04-06-2018, 10:47 AM
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Started by LRU, 04-06-2018, 06:59 AM
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Started by leonardo_ca, 04-05-2018, 12:41 PM
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Started by Jane M, 08-31-2015, 12:59 AM
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Started by Ankita Lawarde, 04-06-2018, 01:07 AM
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Started by dayana42200, 04-02-2018, 03:07 AM
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Started by plabaj, 02-01-2018, 08:42 AM
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
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    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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    06-02-2026, 10:05 AM
  • SEQadmin2
    Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends
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    With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.


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    Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
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