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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Started by laura, 05-09-2011, 04:42 AM
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35 responses
107,335 views
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by laura
12-05-2013, 02:37 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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236 responses
683,082 views
1 reaction
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by ECO
12-25-2009, 06:45 PM
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Started by efoss, 10-14-2011, 01:35 PM
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9 responses
15,616 views
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by 1520191
09-04-2014, 12:44 AM
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Started by MayaAM, 09-03-2014, 11:29 PM
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0 responses
1,159 views
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by MayaAM
09-03-2014, 11:29 PM
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Started by tinguzman, 09-03-2014, 11:05 PM
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0 responses
1,168 views
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by tinguzman
09-03-2014, 11:05 PM
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Started by pepperoni, 09-03-2014, 02:15 PM
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3 responses
1,880 views
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by pepperoni
09-03-2014, 03:39 PM
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Started by pepperoni, 09-03-2014, 01:51 PM
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5 responses
2,197 views
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Started by arnikastern, 09-03-2014, 06:29 AM
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1 response
1,456 views
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by SrCardgage
09-03-2014, 03:16 PM
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Started by papori, 02-09-2012, 04:24 AM
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8 responses
12,886 views
0 reactions
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by tulipnandu
09-03-2014, 02:16 PM
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Started by Sergioo, 09-03-2014, 11:14 AM
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0 responses
1,212 views
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by Sergioo
09-03-2014, 11:14 AM
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TPM vs RPKM
by Benh
Started by Benh, 09-03-2014, 08:46 AM
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3 responses
8,640 views
0 reactions
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Last Post
by Benh
09-03-2014, 09:42 AM
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Started by Helical, 10-11-2013, 04:52 AM
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1 response
1,483 views
0 reactions
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Last Post
by mondongho
09-03-2014, 05:36 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Today, 10:08 AM
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Today, 10:08 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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