In common with most other labs which run Illumina sequencers we make extensive use of a bioanalyzer to do basic library QC and a real time PCR machine to do the quantitation of the libraries prior to sequencing. I guess that similar checks would be appropriate for other sequencing technologies.
Other than that there's nothing which wouldn't be found in any molecular biology lab.
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Equpment Cost in setting up NGS core
Hello all,
I am currently trying to weigh the pros/cons in buying different equipment to support library prep and pre-run quality control for illumina/454/SOLiD as well as possibly upcoming ion torrent etc etc.
So I would like to draw from collective experience and put together a wish list of sorts from people that now routinely run these pipelines. Are there any instruments that you all view as indispensable? What about those that are versatile enough to be helpful for more than one tech?
I'm starting a list, but would like input from all the experts here.
Thanks!
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