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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....

Hello,

Can you please help me understand what is the function of NEBNext adapters (hairpinloop adapters) during library preparation. Please...

Hello,
there are multiple published manners of getting much cheaper library preparations if you do them in-house. That's great if you have a qualified...

Hi All,

I have got an very interesting observation during troubleshooting of an experiment with patient sample as described below (I would...

Hi,

About half our samples post library prep (poly(A) selection) + sequencing had around 50% rRNA content. The samples were re-prepped...

How Fast?
1-2 hours protocols

How Simple?
10 min of hands-on time

What you get:

• Fast protocol
  - 1-1.5

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Library preparation for ancient DNA sequencing is very difficult due to the nature of the DNA. Now the first library prep kit specific for ancient DNA...

Library preparation for ancient DNA sequencing is very difficult due to the nature of the DNA. Now the first library prep kit specific for ancient DNA...

hello everyone,

I have been asked to carry forward someone's project that's on chloroplast DNA isolation from an algae.

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In the field of genomics everybody knows (or at least heard) that sequencing costs dropped dramatically during the last decade. One of the components...

Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2

NGS library preparation is a tedious and time-consuming procedure. It is more complicated and less effective with samples of single stranded DNA or ancient...

I’m going to prepare a sequencing library based on Illumina TruSeq. The protocol my experiment is based on uses single-end sequencing, but a colleague...