Does anyone know of a quicker alternative to PCR/agarose gel when QCing bisulphite conversion in large numbers of samples?
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I'm mostly posting to also follow this thread since i've also struggled with this.
I've tried the Qubit RNA kit, the zymo bisulfite converted control and just ballparking it based on the expected recovery %. Honestly, qPCR setup might be the best high throughput method for quantitation for you but it didn't quite fulfill all of my projects requirements
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
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