I started working on whole genome sequencing and exome sequencing with Illumina HiSeq 2000 and also with 454 sequencer. Can anybody please help me to understand the data analysis of the sequenced reads????
-
-
If you'd like answers to such a question, you should start by posting in the correct forum. Then, explain what you want to do with the reads. You're query can be condensed to "I ran an experiment; how do I analyze it?", and nobody can really help you without some real detail.Originally posted by rd69 View Post -
Anyone remember the days were you just dumped 3 volumes of Maniatis on a student desk and turned around... probably not.Originally posted by rd69 View PostComment
-
Moved this to a new thread...and I really encourage the original poster to read the following:
Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment