What is the depth of coverage on human genome for a HiSeq rapid run versus high output? for a miseq, what is the depth of coverage that can be done on it? thanks in advance. on the older hiseq 2000 and GAIIx what are their depths of coverage?
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Here are published specs for HiSeq: http://www.illumina.com/systems/hise...fications.ilmn
For MiSeq: http://www.illumina.com/systems/mise...fications.ilmn
It is generally possible to get data in that neighborhood as long as your libraries are of good quality. Library quality will also determine the uniformity of coverage.
You can do gross calculation for coverage based on spec numbers above. -
Illumina has a coverage calculator:
As long as you know your genome size, you can use it to calculate coverage on any of their instruments.Comment
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