What is the depth of coverage on human genome for a HiSeq rapid run versus high output? for a miseq, what is the depth of coverage that can be done on it? thanks in advance. on the older hiseq 2000 and GAIIx what are their depths of coverage?
-
-
Here are published specs for HiSeq: http://www.illumina.com/systems/hise...fications.ilmn
For MiSeq: http://www.illumina.com/systems/mise...fications.ilmn
It is generally possible to get data in that neighborhood as long as your libraries are of good quality. Library quality will also determine the uniformity of coverage.
You can do gross calculation for coverage based on spec numbers above. -
Illumina has a coverage calculator:
As long as you know your genome size, you can use it to calculate coverage on any of their instruments.Comment
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment