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What is the depth of coverage on human genome for a HiSeq rapid run versus high output? for a miseq, what is the depth of coverage that can be done on it? thanks in advance. on the older hiseq 2000 and GAIIx what are their depths of coverage?
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Here are published specs for HiSeq: http://www.illumina.com/systems/hise...fications.ilmn
For MiSeq: http://www.illumina.com/systems/mise...fications.ilmn
It is generally possible to get data in that neighborhood as long as your libraries are of good quality. Library quality will also determine the uniformity of coverage.
You can do gross calculation for coverage based on spec numbers above. -
Illumina has a coverage calculator:
As long as you know your genome size, you can use it to calculate coverage on any of their instruments.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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