Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Trying to understand capacity of flow cells for targeted genotyping by sequencing

    Hi everyone,

    I'm relatively new to NGS. I work with a lab that is a part of a larger company that provides genotyping services in the area of PCR and NGS. Typically, for SNP densities less than say 200, we go with PCR and for higher, NGS is more economical.

    The question I have relates to how to calculate how many samples can be run on typical nova seq S2 or S4 flow cells? I'm sure it depends on species, depth, and maybe other factors I'm not thinking of. Is there any resource out there that helps calculate these things or could someone help explain how I can do these calculations?

    Thank you!
    Trey

  • #2
    Hey there TreyWill


    I posted two links below. The first tells you the output of the NovaSeq for each flow cell type. The second resource will help you determine the coverage you would need for your experiment with each Illumina device. There are also a lot of links with the calculator that explains how some of these values are determined.

    I would also note that your output could be different from the ones shown on the site. You should check with your group's previous runs to see their normal output and how much data they expect to get from each run.


    See output, run time, sample throughput, read length, and other NovaSeq 6000 System specifications, and learn how the instrument works.

    Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-27-2024, 06:37 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-27-2024, 06:07 PM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    52 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X