Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Wield MiSeq error rate at the beginning

    Recently when we run amplicon libraries on MiSeqs(2 miSeq instruments), we got wield high error rate dots at the beginning of each read. The overall error rate and Q30 was good, we just would like to understand why this was happening. Did anyonce face this before?

    Thanks

  • #2
    feixiang1019 that's very strange. Have you experienced this with any other libraries you have run?

    Also, what are the amplicons you are sequencing? Is there a lot of diversity in the sequences?

    Comment


    • #3
      We see this all the time with amplicon libraries with low nucleotide diversities. I had the impression this is unavoidable with low diversity libraries.

      Comment


      • #4
        JBKri that's what I was expecting. I haven't run amplicons on an Illumina instrument in a while, but I always remember steep drop-offs when there was low diversity.

        feixiang1019 if this remains an issue you can add more PhiX to your runs or add in more diverse samples.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Best Practices for Single-Cell Sequencing Analysis
          by seqadmin



          While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
          06-06-2024, 07:15 AM
        • seqadmin
          Latest Developments in Precision Medicine
          by seqadmin



          Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

          Somatic Genomics
          “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
          05-24-2024, 01:16 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 06-17-2024, 06:54 AM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-14-2024, 07:24 AM
        0 responses
        21 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-13-2024, 08:58 AM
        0 responses
        17 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-12-2024, 02:20 PM
        0 responses
        20 views
        0 likes
        Last Post seqadmin  
        Working...
        X