Header Leaderboard Ad

Collapse

Wield MiSeq error rate at the beginning

Collapse

Announcement

Collapse

SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
See more
See less
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Wield MiSeq error rate at the beginning

    Recently when we run amplicon libraries on MiSeqs(2 miSeq instruments), we got wield high error rate dots at the beginning of each read. The overall error rate and Q30 was good, we just would like to understand why this was happening. Did anyonce face this before?

    Thanks

  • #2
    feixiang1019 that's very strange. Have you experienced this with any other libraries you have run?

    Also, what are the amplicons you are sequencing? Is there a lot of diversity in the sequences?

    Comment


    • #3
      We see this all the time with amplicon libraries with low nucleotide diversities. I had the impression this is unavoidable with low diversity libraries.

      Comment


      • #4
        JBKri that's what I was expecting. I haven't run amplicons on an Illumina instrument in a while, but I always remember steep drop-offs when there was low diversity.

        feixiang1019 if this remains an issue you can add more PhiX to your runs or add in more diverse samples.

        Comment

        Latest Articles

        Collapse

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 01:08 PM
        0 responses
        6 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-01-2023, 08:56 PM
        0 responses
        12 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-01-2023, 07:33 AM
        0 responses
        140 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-31-2023, 07:50 AM
        0 responses
        178 views
        0 likes
        Last Post seqadmin  
        Working...
        X