JBKri that's what I was expecting. I haven't run amplicons on an Illumina instrument in a while, but I always remember steep drop-offs when there was low diversity.
feixiang1019 if this remains an issue you can add more PhiX to your runs or add in more diverse samples.
-
We see this all the time with amplicon libraries with low nucleotide diversities. I had the impression this is unavoidable with low diversity libraries.Leave a comment:
-
feixiang1019 that's very strange. Have you experienced this with any other libraries you have run?
Also, what are the amplicons you are sequencing? Is there a lot of diversity in the sequences?Leave a comment:
-
Wield MiSeq error rate at the beginning
Recently when we run amplicon libraries on MiSeqs(2 miSeq instruments), we got wield high error rate dots at the beginning of each read. The overall error rate and Q30 was good, we just would like to understand why this was happening. Did anyonce face this before?
Thanks
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
Leave a comment: