Retrieve molecular barcodes

[Polly]

Hello everyone,

I'm new to NGS and I have a hard time with molecular barcodes (UMIs). Does anyone know where I can get the pattern for my molecular barcodes, so that I can extract them with UMI-tools extract ? The only information I found in the guide of the sequencing kit is that it is an 8 nucleotide sequence.

Thank you for your help.

[Ben3]

Polly what kits did you use? I can usually find the sequences but only if I know the specific library kits used and the you used a specific variation of the kit. Sometimes the UMIs and indexes will say things like "set A" which is also important to know.

[Polly]

It was the Agilent Sureselect Human all exon V7 kit.

[Ben3]

Polly I've tried searching across their site and couldn't seem to find anything. I know some companies will make you message them to get the sequence information you need. Maybe you can email Agilent's support and ask for the csv file that contains the UMI sequences.

[Polly]

Thank you very much Ben3, I will ask them !