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  • oursu
    Junior Member
    • Mar 2014
    • 2

    #1

    bamToBed bedpe

    Hi,

    I m running bamToBed using bedtools/2.18.0.
    When I use the -bedpe option, I am getting an incomplete file, in particular, of my total 50 million reads (25M pairs), I am only getting ~400 000 in the bedpe file.

    I want to ask for some advice about how to solve this problem.

    I have tried
    1) increasing the allocated memory for the process
    2) checked whether I have both reads beloning to each pair in my bam file (I do)
    3) omitting the -bedpe option, I get the correct bed file with 50 million reads in it
    4) i looked at the location in the -n sorted bam where the writing stops, and I could not find any pattern to explain my observation

    Thank you
    Last edited by oursu; 03-29-2014, 07:21 PM.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Are you use an 32bit system?

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