Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • oursu
    Junior Member
    • Mar 2014
    • 2

    #1

    bamToBed bedpe

    Hi,

    I m running bamToBed using bedtools/2.18.0.
    When I use the -bedpe option, I am getting an incomplete file, in particular, of my total 50 million reads (25M pairs), I am only getting ~400 000 in the bedpe file.

    I want to ask for some advice about how to solve this problem.

    I have tried
    1) increasing the allocated memory for the process
    2) checked whether I have both reads beloning to each pair in my bam file (I do)
    3) omitting the -bedpe option, I get the correct bed file with 50 million reads in it
    4) i looked at the location in the -n sorted bam where the writing stops, and I could not find any pattern to explain my observation

    Thank you
    Last edited by oursu; 03-29-2014, 07:21 PM.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Are you use an 32bit system?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    32 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    44 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Working...