Hi all,
We constructed a library, using 140-160bp cut fragment as recommended by illumina. We did a 1X50bp run. We compared it with HiSeq data we obtained earlier.
1. In all cases, the HiSeq is producing a higher number of 21nt than 24nt but the reverse is true for the MiSeq run. Is there any technical reason for this bias?
2. We observe a peak at 32-33nt in the MiSeq. what it represents? Any suggestions?
Thanks for your reply.
We constructed a library, using 140-160bp cut fragment as recommended by illumina. We did a 1X50bp run. We compared it with HiSeq data we obtained earlier.
1. In all cases, the HiSeq is producing a higher number of 21nt than 24nt but the reverse is true for the MiSeq run. Is there any technical reason for this bias?
2. We observe a peak at 32-33nt in the MiSeq. what it represents? Any suggestions?
Thanks for your reply.
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