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"Next Generation" does have a nicer ring to it than "Markedly Faster than Last Year (but wait until next year)"
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Winner!Originally posted by krobison View Post
SEQanswers: The Markedly Faster than Last Year Sequencing CommunityComment
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I like it, too. "All samples will undergo Markedly Faster Than Last Year sequencing to identify rare variants".Comment
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the acronym is not the sexiest though..Comment
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LBP sequencing
OK, so how about LBP (little bitty pieces) sequencing?Comment
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"All samples will undergo Markedly Faster and Slightly Longer Than Last Year sequencing to identify rare variants".Comment
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Which generation?
By my count Sanger is 3rd generation. The 1st nucleic acid sequence I remember was a tRNA sequence obtained by analysis of nuclease products on 2D thin layer chromatography. Then Maxam-Gilbert chemical sequencing emerged as the 1st tractable way to sequence longer stretches of DNA (how many or you know how to pour a meter long, 0.5mm thick polyacrylamide gel with no bubbles?). Then came the Sanger method which was much less finicky than chemical sequencing and ruled for more or less a human generation (albeit with technical improvements like switching to capillary electrophoresis along the way).
Now what you have is the parallel evolution of a variety of different technologies. I think the generational analogy has broken down. Perhaps a speciation analogy is more applicable now. I don't see single molecule sequencing as the next wave per se, rather each technology competing now has a niche application and often two technologies need to be combined to do a particular job well.Comment
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How about Age of Aquarius Sequencing? We could all grow our hair out, it'd be groovy.Comment
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Oh, the gels I have poured....
I did not have any bubble problems with acrylamide gels--would simply degas a solution of 5x recrystalized acrylamide before pouring....
Additionally, I've made purified whole protein samples, but never got to perform sequencing on those. Protein sequencing provided us the first glimpse at evolutionary conservation and those isoforms in action. Now we have the short course.
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It will be "XGen": Before X PRIZE, After X PRIZE
How to identify sequencing technologies will become straightforward after some team demonstrates they can win the $10M Archon Genomic X PRIZE <http://genomics.xprize.org/>. The stringent requirements of the AGXP will distinguish all platforms capable of achieving such goals and henceforth sequencing platforms would be identified as “X PRIZE Capable” versus those that are not.
Larry Kedes
Senior Advisor. X PRIZE Foundation
Scientific Director, Archon Genomic X PRIZEComment
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I like the idea of always having the "next gen" name.Originally posted by Michael.James.Clark View Post
ECO, I actually think you might be okay leaving "next generation" in the tag line. I mean, we are always going to be discussing the "next generation" (plus some of the last generation, probably).
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Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM -
Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....02-21-2023, 02:14 PM
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