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Hi Everyone,
I am having some trouble using usearch ublast. I am trying test a database I made using:
usearch -makeudb_ublast myFasta.fasta -output db.udb -alpha nt
I used a sequence that should return hits, but usearch ublast says that 0.0% matched. The command I am currently using is:
usearch -ublast query.fasta -db db.udb -strand both -evalue 1e-5 -alnout output.aln
I have tried using different combinations of -evalue and -strand and I have double checked that I am using the command correctly.
Also I made databases with makeblastdb, and tests for those returned the proper results when I used the same sequences as in usearch.
I am using usearch v8.1.1861
Can anyone figure out what I am doing wrong?
I am having some trouble using usearch ublast. I am trying test a database I made using:
usearch -makeudb_ublast myFasta.fasta -output db.udb -alpha nt
I used a sequence that should return hits, but usearch ublast says that 0.0% matched. The command I am currently using is:
usearch -ublast query.fasta -db db.udb -strand both -evalue 1e-5 -alnout output.aln
I have tried using different combinations of -evalue and -strand and I have double checked that I am using the command correctly.
Also I made databases with makeblastdb, and tests for those returned the proper results when I used the same sequences as in usearch.
I am using usearch v8.1.1861
Can anyone figure out what I am doing wrong?