Hi,
I have been following the github tutorial, https://github.com/griffithlab/rnaseq_tutorial/wiki
to learn RNAseq.
I was on the indexing step and it says to first export exons and splice sites from reference genome using in-built python scripts before starting the indexing. And then that this information will be used during alignment.
It would be great if someone could explain the rationale behind this step.
I have been following the github tutorial, https://github.com/griffithlab/rnaseq_tutorial/wiki
to learn RNAseq.
I was on the indexing step and it says to first export exons and splice sites from reference genome using in-built python scripts before starting the indexing. And then that this information will be used during alignment.
It would be great if someone could explain the rationale behind this step.
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