Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Alternative to Qiagen Puregene Tissue kit for HMW gDNA?

    Hello,

    We're extracting high molecular weight gDNA for PacBio from an insect-a bee- using the Qiagen Puregene Tissue kit (4g). We have trouble with this kit back-ordered all the time. Could someone from the community recommend an alternative for HMW gDNA that works well on tissue for PacBio?

    Thanks,

    Dacotah

  • #2
    I have tried the following protocol with insects and it works quite well in terms of purity and length:

    Modified low‐salt CTAB extraction of high‐quality DNA from contaminant‐rich tissues



    A benchtop protocol is available in the "Supporting Information" section.

    Comment


    • #3
      Hi Dacotah,

      Qiagen's PureGene kits are nearly identical to the former Epicentre MasterPue DNA Purification kits, which are now available through Lucigen/Biosearch Technologies. Catalog numbers would be MC85200 for 200 reactions and MC89010 for 10 reactions. Both are non-column based products.

      Olafblue1955

      Comment


      • #4
        Oh, that's great, thank you very much.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM
        • seqadmin
          Techniques and Challenges in Conservation Genomics
          by seqadmin



          The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

          Avian Conservation
          Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
          03-08-2024, 10:41 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 06:37 PM
        0 responses
        11 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, Yesterday, 06:07 PM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-22-2024, 10:03 AM
        0 responses
        51 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-21-2024, 07:32 AM
        0 responses
        68 views
        0 likes
        Last Post seqadmin  
        Working...
        X