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If anyone uses big dye in their sanger sequencing reactions, I have a question. After big dye is added to your sequencing reaction, how long can that reaction be kept at 4 deg C?
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Hey seqgirl,
This question is not really appropriate for this forum...but I'll leave it for now.
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I've kept sequencing reactions overnight before at 4 degrees C and they have been fine but it's probably not a good idea to do it routinely.
Scott.Comment
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hi friends
we are working with ABI ABI 3130xl genetic analyzer. we used to use BigDye Kit . but we dont have it anymore and we want to use alternative. does anybody knows that what we can use as alternative for BigDye Kit? are there ready labelled ddNTPS available that can be used for ABI ABI 3130xl genetic analyzer.? or are there any alternatie for their original ddNTPs?
please help me
please help me
please help me
My Job depend on this
I will loose my job if I dont find a soloutionComment
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Old-school, certainly the wrong forum though try ABRF instead.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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