If anyone uses big dye in their sanger sequencing reactions, I have a question. After big dye is added to your sequencing reaction, how long can that reaction be kept at 4 deg C?
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Hey seqgirl,
This question is not really appropriate for this forum...but I'll leave it for now.
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I've kept sequencing reactions overnight before at 4 degrees C and they have been fine but it's probably not a good idea to do it routinely.
Scott.Comment
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hi friends
we are working with ABI ABI 3130xl genetic analyzer. we used to use BigDye Kit . but we dont have it anymore and we want to use alternative. does anybody knows that what we can use as alternative for BigDye Kit? are there ready labelled ddNTPS available that can be used for ABI ABI 3130xl genetic analyzer.? or are there any alternatie for their original ddNTPs?
please help me
please help me
please help me
My Job depend on this
I will loose my job if I dont find a soloutionComment
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Old-school, certainly the wrong forum though try ABRF instead.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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