Hi all,
I'm new in this field and want to learn something here.![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?
Thanks
-Flora
I'm new in this field and want to learn something here.
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?
Thanks
-Flora