Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • floraliu
    started a topic Hi, one question about RNA-Seq library prep

    Hi, one question about RNA-Seq library prep

    Hi all,

    I'm new in this field and want to learn something here.

    I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?


    Thanks
    -Flora
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR
    by seqadmin



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
ASHG 2024 Highlights – Part Two by seqadmin
Started by seqadmin, Today, 11:09 AM
0 responses
22 views
0 likes
 Last Post seqadmin
by seqadmin
Today, 11:09 AM  
ASHG 2024 Highlights – Part One by seqadmin
Started by seqadmin, Today, 06:13 AM
0 responses
20 views
0 likes
 Last Post seqadmin
by seqadmin
Today, 06:13 AM  
Seq-Scope Expands Possibilities for High-Resolution Gene Expression Analysis by seqadmin
Started by seqadmin, 11-01-2024, 06:09 AM
0 responses
30 views
0 likes
 Last Post seqadmin
by seqadmin
11-01-2024, 06:09 AM  
New Model Aims to Explain Polygenic Diseases by Connecting Genomic Mutations and Regulatory Networks by seqadmin
Started by seqadmin, 10-30-2024, 05:31 AM
0 responses
21 views
0 likes
 Last Post seqadmin
by seqadmin
10-30-2024, 05:31 AM  
View All
Working...
X