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  • floraliu
    Junior Member
    • May 2011
    • 1
    #1

    Hi, one question about RNA-Seq library prep

    Hi all,

    I'm new in this field and want to learn something here.

    I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?


    Thanks
    -Flora
    Tags: None
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