Hi all!
I'm a PhD-student in Germany and work with small eukaryotes that thrive within ocean currents (Plankton). In one of my projects we sequence (Illumina Miseq) amplicons from more than 50 samples to gain a picture about the diversity of protists in the water column.
Since I haven't work with Illumina before, I have a plenty of questions for which I hope to find answers in this forum.
One urgent question (and stupid I guess.. as it is always :
Can you work with paired-end reads when only one Primer (Reverse) was labeled? Is a correct assembling of the reads and their assignment to the different samples possible? Even if only read1 can be related to one sample, it doesn't matter were read2 originates, or? .. The main thing is, that there is enough overlapping (e.g. 90 bp) and no reads are left.. or am I totally wrong?
I am confused... hope you can help!
Paralia
I'm a PhD-student in Germany and work with small eukaryotes that thrive within ocean currents (Plankton). In one of my projects we sequence (Illumina Miseq) amplicons from more than 50 samples to gain a picture about the diversity of protists in the water column.
Since I haven't work with Illumina before, I have a plenty of questions for which I hope to find answers in this forum.
One urgent question (and stupid I guess.. as it is always :
Can you work with paired-end reads when only one Primer (Reverse) was labeled? Is a correct assembling of the reads and their assignment to the different samples possible? Even if only read1 can be related to one sample, it doesn't matter were read2 originates, or? .. The main thing is, that there is enough overlapping (e.g. 90 bp) and no reads are left.. or am I totally wrong?
I am confused... hope you can help!
Paralia
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