Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • More Accurate Tuberculosis Genome

    A team of researchers from Rutgers University updated the reference sequence for Mycobacterium tuberculosis strain H37Rv in a recent publication in Nature Communications.

    The previous reference sequence for M. tuberculosis, the causative agent of tuberculosis, was commonly used in research despite containing inaccuracies. Tuberculosis is a leading cause of death worldwide and causes illness for millions of people each year (World Health Organization). Therefore, the availability of an accurate reference genome was crucial for clinical and infectious disease research.

    Assembly of a complete M. tuberculosis genome was challenging due to the organism’s highly repetitive regions, which account for around 10% of the genome. These difficult regions also make an assembly using short reads much more difficult. To combat these challenges, the researchers used the MinION from Oxford Nanopore to obtain the needed long reads and polished the assembly using short reads from Illumina instruments.

    The final assembly of strain H37Rv added 6.4 kb of new sequence into the reference along with changes to 10 major regions. It also led to the development of Bact-Builder, a new tool for de novo assembly of accurate bacterial genomes.

    Bact-Builder uses long reads for a consensus-based assembly and further polishes the assembly with both long and short reads. It was first designed using reads generated in silico and was then tested using actual reads from the MinION and Illumina’s NovaSeq.

    The creation of Bact-Builder can help researchers build other bacterial genomes and the changes to the H37Rv reference sequence hope to improve tuberculosis research worldwide.

    Read the original journal article here.

Latest Articles

Collapse

  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
    by seqadmin




    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
    07-08-2024, 03:19 PM
  • seqadmin
    Exploring Human Diversity Through Large-Scale Omics
    by seqadmin


    In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
    06-25-2024, 06:43 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 11:09 AM
0 responses
15 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-19-2024, 07:20 AM
0 responses
148 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-16-2024, 05:49 AM
0 responses
121 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-15-2024, 06:53 AM
0 responses
111 views
0 likes
Last Post seqadmin  
Working...
X