Hi all,
Sorry, my english is not perfect, but I can try to explain my question.
I want to sequence an amplicon by undirectional sequencing. I was looking the "Guidelines for amplicon experimental design" and that says I have to use Primers A and B (Lib-L) to amplify the DNA, but the sequencing will be just using Primer A.
So, Primer B will be used just to bind the DNA to the capture beads? Or exists another function for Primer B in this case (unidirectional sequencing)?
Can I forget the primer B and just design my fusion primers using only Primer A?
Thanks for your help!
Sorry, my english is not perfect, but I can try to explain my question.
I want to sequence an amplicon by undirectional sequencing. I was looking the "Guidelines for amplicon experimental design" and that says I have to use Primers A and B (Lib-L) to amplify the DNA, but the sequencing will be just using Primer A.
So, Primer B will be used just to bind the DNA to the capture beads? Or exists another function for Primer B in this case (unidirectional sequencing)?
Can I forget the primer B and just design my fusion primers using only Primer A?
Thanks for your help!
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