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Hello all!
Since we had a Titanium upgrade I had only few of successful runs. Lately I'm experiencing with reducing number of detected wells. For my good runs I had 2.1, 2.2 millions wells. But lately I had only 1.5, 0.6, 1.1 millions wells. According to the manual we have to use minifuge to pellet the beads. But Roche representative suggested to use bechtop centrifuge at 16g since now we have smaller beads. I think at this g-force beads can stick together and do not fit into the wells on the plate. And this is what I experience: when I load the plate with DNA beads, after centrifugation the supernatant still milky. Does anybody have same problem?
Also during my last runs I had a lot of short CACACACACA....and its complement TGTGTGTGTG... , and some other sequences. Is this some sort of contamination or some artifact?
Thanks!
Since we had a Titanium upgrade I had only few of successful runs. Lately I'm experiencing with reducing number of detected wells. For my good runs I had 2.1, 2.2 millions wells. But lately I had only 1.5, 0.6, 1.1 millions wells. According to the manual we have to use minifuge to pellet the beads. But Roche representative suggested to use bechtop centrifuge at 16g since now we have smaller beads. I think at this g-force beads can stick together and do not fit into the wells on the plate. And this is what I experience: when I load the plate with DNA beads, after centrifugation the supernatant still milky. Does anybody have same problem?
Also during my last runs I had a lot of short CACACACACA....and its complement TGTGTGTGTG... , and some other sequences. Is this some sort of contamination or some artifact?
Thanks!