emPCR Reagents

[Anthony.287]

I'm back with another question!
I was wondering if the emPCR reagents are the same in both the Lib-L and Lib-A kits. Not the primers or beads, of course, but things like the Capture Bead Wash buffer, emPCR additive, enzyme mix, PPiase, etc. There have been a few occasions when I've set up Lib-L and Lib-A emulsions at the same time, and it would be handy if I could use the same reagents for both.
Thank you!!

[RCJK]

I would think so, but don't know for sure. Maybe check the part number of the individual tubes and see if they match?

[Nicole 454 Sequencing]

Hi Anthony,

The Amplification Primer, Enrichment Primer, Sequencing Primer and Capture beads are unique to the Lib-A and Lib-L kits, so be sure not to interchange these. The other reagents are common between the two kits and could be used during a parallel set-up.

Nicole

Technical Support Scientist
454 Life Sciences, A Roche Company