Hi guys.
I've been having problems recently with the Roche/454 amplification primers mis-priming my amplicon templates in regions with some weak complimentarity. This is really messing up my data by creating abortive, short DNA fragments on my beads.
I'm trying to contact Roche about this, but I'd love to get a sense is this is something other people who are doing Lib-A sequencing have seen before. Since I've seen these emPCR artifacts occur three times in the last month I'm assuming it can't be an isolated problem here. The more instances of I can show of this problem perhaps the more support Roche/454 will be willing to provide.
I've been having problems recently with the Roche/454 amplification primers mis-priming my amplicon templates in regions with some weak complimentarity. This is really messing up my data by creating abortive, short DNA fragments on my beads.
I'm trying to contact Roche about this, but I'd love to get a sense is this is something other people who are doing Lib-A sequencing have seen before. Since I've seen these emPCR artifacts occur three times in the last month I'm assuming it can't be an isolated problem here. The more instances of I can show of this problem perhaps the more support Roche/454 will be willing to provide.
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