I have problem with XL+ machine. Since we've got an upgrade last fall, we have not been able to get a good run. Our first run of control beads gave us about 740 bp average read length, and since we are having problems, recently we did another control beads run which gave us about 650bp average length. Our samples consistently produce 450-470 average length. My initial thought was that 1600-1800 recommended length of library is too high. So I prepared two sets of libraries with 1100 and 1500 average length, did emPCR, collected supernatant after first denaturation step, concentrated it and ran on BioAnalyzer. The pictures are attached. The conclusion is that there is very small deference between 1100, 1500 and 1800 bp libraries. But it does not bring me closer to the solution of our problem.
Does anybody have a similar problem? What is the way to overcome?
Any thoughts?
Does anybody have a similar problem? What is the way to overcome?
Any thoughts?
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