Rapid Library titration range with qPCR?

[Anthony.287]

Good morning!
I was wondering if anyone has suggestions for a titration range of Rapid libraries quantified with qPCR instead of RFU. I've been using the qPCR for amplicon libraries and have a good titration range for those, but haven't done a rapid prep in some time. Any suggestions?
Thank you!!

[RCJK]

I'd say try 0.1-0.4cpb. Most of my rapid libraries for either XLR70 or XL+ have had good enrichments in this range. For a titration maybe throw one in at 0.8-1.0cpb.
I'd use a little bit lower cpb for MV rxns as well as they seem to have higher enrichments than the SV or LV at comparable inputs.

[Anthony.287]

Thank you!
I used a range similar to the one I've been using with amplicons (0.05-0.5), and I'll know within a few hours how/if it worked.

[Anthony.287]

Well, it didn't work. At all. One of the 16 samples might have had a little bit of enriched beads, but not enough to sequence. Does anyone know if there are bad reagents lots floating around?