Hello to everyone here, this is my first question in this forum. and thanks in advance of replying. I just got the RNA-seq data by 454 GS-FLX (sff, fna and qual files).my question is :
1.Whether the data include the sequence of 20 bp of emotion PCR primer and 20 bp of sequencing, 4bp of "key sequence" as well?
2. Which kind of assembly method will be used by the data provider in this case?
1.Whether the data include the sequence of 20 bp of emotion PCR primer and 20 bp of sequencing, 4bp of "key sequence" as well?
2. Which kind of assembly method will be used by the data provider in this case?