Hi there!,
I suscribe to this forum cause I notice that is really useful for everyone who is working un NGS technology.
Well, my trouble starts with de gap closing of a phage... there are just 6 contigs but the trouble is that there is not references to use to make primers or orient the postition of them.
I tried to use GapResolution software, but newbler 2.6 doesn't give me 454Scaffold.txt file althought I selected the option "output scaffolds" and if I don't have this, I can't do anything with it.
I tried to desing primers with a pair of softwares, but a contig is as little that it doesn't give primers.
I don't know what strategy to take... Please I need your help.
greetings
I suscribe to this forum cause I notice that is really useful for everyone who is working un NGS technology.
Well, my trouble starts with de gap closing of a phage... there are just 6 contigs but the trouble is that there is not references to use to make primers or orient the postition of them.
I tried to use GapResolution software, but newbler 2.6 doesn't give me 454Scaffold.txt file althought I selected the option "output scaffolds" and if I don't have this, I can't do anything with it.
I tried to desing primers with a pair of softwares, but a contig is as little that it doesn't give primers.
I don't know what strategy to take... Please I need your help.
greetings
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