I thought the new "scaffold" option in Newbler might do something interesting with my shotgun data even though I thought this term was previously only discussed in relation to paired-end data. Anyway, I have tried doing a de novo assembly of shotgun data both with and without the scaffold option selected. It seems that the scaffold output is identical to the largest contigs (of course there is variation depending upon scaffold and large contig length cutoffs). Is this normal or should I have expected anything different?
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by seqadmin
The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
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Channel: Articles
01-24-2023, 01:19 PM -
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by seqadminSingle-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...-
Channel: Articles
01-09-2023, 03:10 PM -
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