I thought the new "scaffold" option in Newbler might do something interesting with my shotgun data even though I thought this term was previously only discussed in relation to paired-end data. Anyway, I have tried doing a de novo assembly of shotgun data both with and without the scaffold option selected. It seems that the scaffold output is identical to the largest contigs (of course there is variation depending upon scaffold and large contig length cutoffs). Is this normal or should I have expected anything different?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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