Hi everyone! I am new in the forum. So, maybe my answer is extremely basic.
I want to design primers for a 454 experiment, but I have some questions.
Firstly, is it my design correct?
![](http://i.imgur.com/UjJcC.jpg)
Secondly...the sequences of the MIDs in A and B primers should be the same? complement? o totally different?
Thanks in advance
I want to design primers for a 454 experiment, but I have some questions.
Firstly, is it my design correct?
![](http://i.imgur.com/UjJcC.jpg)
Secondly...the sequences of the MIDs in A and B primers should be the same? complement? o totally different?
Thanks in advance
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