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Hello everybody. I am new here, so I apologize if this is posted in the incorrect forum. Please feel free to direct me elsewhere if required.
I have been using the GS Junior platform for 6 months and have encountered my first technical problem. After starting my last sequencing run (only a few hours before I was to leave on vacation!) I got an error messge stating: "Failure Calibrating Flow Rate. Sequencing in Progress." The sequencing run appeared to start as normal and got to cycle 2 where it stalled for 4.5 hours. I ended up aborting the run as I was heading on vacation for 10 days and didn't want to risk breaking the machine. We replaced the tubing on the GS Junior, although I am doubtful that was the issue as only 20 runs have been performed since it was last replaced, and Roche recommends 100 runs is when it should be replaced. Roche installed the new software two weeks ago, and also (apparently) performed a Preventative Maintenance at that time. I have performed one successful run with the new software.
Any ideas what could be happening? I am reluctant to put another sequencing run on until I look into this further as it is an expensive way to learn by trial and error.
I have been using the GS Junior platform for 6 months and have encountered my first technical problem. After starting my last sequencing run (only a few hours before I was to leave on vacation!) I got an error messge stating: "Failure Calibrating Flow Rate. Sequencing in Progress." The sequencing run appeared to start as normal and got to cycle 2 where it stalled for 4.5 hours. I ended up aborting the run as I was heading on vacation for 10 days and didn't want to risk breaking the machine. We replaced the tubing on the GS Junior, although I am doubtful that was the issue as only 20 runs have been performed since it was last replaced, and Roche recommends 100 runs is when it should be replaced. Roche installed the new software two weeks ago, and also (apparently) performed a Preventative Maintenance at that time. I have performed one successful run with the new software.
Any ideas what could be happening? I am reluctant to put another sequencing run on until I look into this further as it is an expensive way to learn by trial and error.