Hi,
does anyone experienced a high fraction of really short reads in a Titanium run ?
We have sequenced genomic samples from two fish with the 454 Titanium (1/2 plate per fish) and about 30 - 40 % of all reads are shorter than 100 bps, in both cases. However the rest of the reads looks all really good with lengths of 500 bps and more ...
I have attached the read profile. Has anyone seen such a bimodal distribution and has an idea what the problem could be ? Library prep ? Sequencing ?
Thanks,
Andreas
does anyone experienced a high fraction of really short reads in a Titanium run ?
We have sequenced genomic samples from two fish with the 454 Titanium (1/2 plate per fish) and about 30 - 40 % of all reads are shorter than 100 bps, in both cases. However the rest of the reads looks all really good with lengths of 500 bps and more ...
I have attached the read profile. Has anyone seen such a bimodal distribution and has an idea what the problem could be ? Library prep ? Sequencing ?
Thanks,
Andreas
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