I got a bacteria genome which sequencing by 454 technique and Illumina technique separately.
After run 454 reads by using MIRA and BAMBUS, I extract the longest scaffold and treat it as a backbone. I hope that I able to polish this longest scaffold by using Illumina reads to get better assembly result.
I just confusing how to use MIRA to polish my longest scaffold and get better assembly result.
Thanks again for everybody.
-
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM -
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...09-09-2024, 10:59 AM