Hello,
I am trying to figure out how to run an assisted assembly with runAssembly. I have been told that you can give runAssembly fasta files in addition to the sff files you want to assemble. I have a reference fasta and I tried giving this to run assembly in addition to the sff files and it skipped it:
Warning: Long read Contig3893 (27001 bp) too long to be assembled. Skipping...
Anyway to get runAssembly to assemble this? I suppose I could shred the fasta into shorter sequences into a multi fasta but that would somewhat defeat the purpose of doing an assisted assembly. Any help is appreciated.
I am trying to figure out how to run an assisted assembly with runAssembly. I have been told that you can give runAssembly fasta files in addition to the sff files you want to assemble. I have a reference fasta and I tried giving this to run assembly in addition to the sff files and it skipped it:
Warning: Long read Contig3893 (27001 bp) too long to be assembled. Skipping...
Anyway to get runAssembly to assemble this? I suppose I could shred the fasta into shorter sequences into a multi fasta but that would somewhat defeat the purpose of doing an assisted assembly. Any help is appreciated.
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