Hello all,
I'm working on a transcriptome which is sequenced by Roche 454. At first I used to assemble it using Newber but the result was awful. Then I tried iAssembler and got a result that looks all right. But when I mapped reads back, only about 30% reads could be located on the assembly.
Is there anyone who can help me with this issue? Many thanks.
I'm working on a transcriptome which is sequenced by Roche 454. At first I used to assemble it using Newber but the result was awful. Then I tried iAssembler and got a result that looks all right. But when I mapped reads back, only about 30% reads could be located on the assembly.
Is there anyone who can help me with this issue? Many thanks.
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