Header Leaderboard Ad


Leading and Lagging strand dephasing in sequencing.



No announcement yet.
  • Filter
  • Time
  • Show
Clear All
new posts

  • Leading and Lagging strand dephasing in sequencing.

    I'm studying the sequencing method as used by different sequenceing technology but i didn't understand the dephasing of the leading and lagging strands

  • #2
    Dephasing in sequencing usually refers to a situation (using Illumina as an example), where the different copies of a DNA fragment within a cluster get out of synch. If a cluster has 10,000 copies of the DNA fragment, these copies all incorporate a fluorescent nucleotide which is imaged. This nucleotide is then "de-blocked" to allow the next nucleotide to be incorporated. If the deblocking doesn't occur on a particular strand, then that strand won't incorporate the next nucleotide. But it might be deblocked in the next round, in which case it will be one base behind the rest of the cluster. As the rounds of incorporation and deblocking progress, more and more of the strands in the cluster get out of synch. The sequences might look like:

    C ATGT
    CAT GT
    At this point, the imaging of the cluster will result in a mix of A and T, so the quality will be low for the basecall.
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com


    Latest Articles


    • seqadmin
      A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
      by seqadmin

      ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

      01-24-2023, 01:19 PM
    • seqadmin
      Introduction to Single-Cell Sequencing
      by seqadmin
      Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

      The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
      01-09-2023, 03:10 PM