Over the past few months I keep getting errors on the sequencer that say PTP heater temperature out of range. Those runs always look bad with no control reads and sample reads under 100 in length. Roche keeps sending someone to try and fix it, they adjust sensors but it doesn't seem like they really know what is wrong and it keeps happening. Has this happened to anyone else? If so how did it get fixed?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM