Originally posted by LFMar
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hi
can anyone tell me how much time does it take to analyse data generated by NGS? I am doin PhD, so just curious to know?
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NGS data analysis
Thank u so much for the manual.......
can anyone tell me how much time (approx) does it take to analyse the NGS generated data..
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Originally posted by vidushi View Posthi
i also need a copy of the 454 Titanium Sequencing Manual please? could you kindly send it to me. It will be a great help for me.
email: [email protected]
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hi
i also need a copy of the 454 Titanium Sequencing Manual please? could you kindly send it to me. It will be a great help for me.
email: [email protected]
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If they've just recently expired, they'd still work and you'll be okay.
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Expired adaptors
Has anyone used adaptors with expired date for 454 rapid library preparation? I've bought the adaptors (up to 10 samples) but I used them for just 2 DNA samples, now I have 8 more samples for library prep and the adaptors date just expired. I don't know if I try to use them or if I buy new ones.
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i will use 454 for transcriptome sequencing of ploids
so if anybody have done such work please give me some suggestions
thank you very much
EMAIL: [email protected]
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is there any protocol for library preparation when transcriptome sequencing using 454?
thx
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About number of samples
Hi all,
I need informations,
What's the maximum number of samples that I could put inside a 454 GS Junior's microtiter plate ?
Thanks
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cDNA sequencing
Many thanks to Bertie and mccallumj. The info are indeed very helpful!
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re Poly A etc
We have had very nice results from Ti sequencing of normalised cDNA prepared using Evrogen Mint + Trimmer kits, using a modified 3' Primer XXXXXXXXXXX(T)4G(T)9C(T)10VN as described by Beldade et al.
The information posted at http://www.cees.uio.no/research/faci...equencing.html was very useful.
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