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  • NEBNext Quick DNA Sample Prep - buying individual enzymes separately

    NEB is selling 2 kits to prepare 454 libraries using dA-tailed ligation:

    1. NEBNext Quick DNA Sample Prep Master Mix Set 2
    2. NEBNext Quick DNA Sample Prep Reagent Set 2

    In the first kit they give the actual enzyme concentrations in the mix, except for the Quick ligase. To decrease the price in preparing 20 libraries, I want to order the NEB enzymes separately. Does anyone have experience in doing it and how much of the Quick ligase (NEB cat. n. M2200S) you use?

    Thanks.

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