Tweet
Hi forum members,
I am just getting my feet wet with sequence analysis, analyzing a small set of cDNA data from a 454 Junior run. I did a successful assembly on the GS de novo assembler and now I am wondering about input parameters such as seed step, seed length, etc. I understand what these are conceptually, but I am wondering how I would want to change them from the defaults and why. In other words, what is the ultimate goal with an assembly and how do these inputs affect it. Fewer contigs/isotigs? Or...?
I am just getting my feet wet with sequence analysis, analyzing a small set of cDNA data from a 454 Junior run. I did a successful assembly on the GS de novo assembler and now I am wondering about input parameters such as seed step, seed length, etc. I understand what these are conceptually, but I am wondering how I would want to change them from the defaults and why. In other words, what is the ultimate goal with an assembly and how do these inputs affect it. Fewer contigs/isotigs? Or...?